The UFC did not announce whether the fight would continue, but the two fighters were introduced and faced off at the ceremonial weigh-ins later on Friday.
Yoel Romero weighed in at 185.2 pounds at his second attempt to make championship weight.
The event can remain a PPV, however, as UFC interim welterweight title contenders Rafael Dos Anjos and Colby Covington both made championship weight for their bout.
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Later, though, despite Romero missing weight on his second attempt, UFC boss Dana White confirmed that the main event will go ahead. On Friday, he came in at 185.2 for his title fight with Robert Whittaker, forcing it to be a non-title fight affair.
UFC veteran and Chicago's own, Clay Guida vs submission specialist, Charles Oliveira. Romero was granted two hours to see if he could lose that last pound but did not show up until 10 minutes before the cut-off point.
The Australian has finished opponents on four occasions during his win streak, and has earned Performance of the Night bonuses with each of his past three knockout wins, including a second-round TKO of Ronaldo Souza as a plus-225 bet at UFC on Fox 24.
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Whittaker initially won the middleweight title with a unanimous decision defeat of Romero at UFC 217.
"I think you do have to get on with it and move on, that's the inevitable outcome", Whittaker told ESPN. Romero earned a third-round KO victory as a plus-150 underdog, but was deemed ineligible to claim the belt after failing to make weight.
Chief among the issues was Saturday's UFC 225 main event.
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Romero was supposed to fight Luke Rockhold for the interim middleweight title at UFC 221.