Explosive allegations from whistleblowers that Crown Casino tampered with poker machines, ignored domestic violence, and undermined government efforts to track money laundering have been tabled in Federal Parliament.
Mr Wilkie said the former employees alleged that while the allegations were central to Crown's Melbourne operations they went further to operation of the entire poker machine industry across the country.
The whistleblowers allege that staff were told to alter poker machines, known in Australia as "pokies", to remove some betting options.
But staff alleged they were instructed to use tools to shave down buttons on new machines to create space for punters to wedge something in the button, so it could be held in place and keep the machine playing without buttons being pressed.
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Wilkie tabled the allegations, including a 30-minute recorded interview with the three whistleblowers, in what is believed to be the first case of video evidence being tabled in federal parliament.
Independent lawmaker and anti-gambling campaigner Andrew Wilkie used parliamentary privilege, which allows lawmakers to make sensitive allegations without legal repercussions, to make the video public and call for an investigation by authorities.
"We take any claims of this type extremely seriously and they will be thoroughly investigated", the commission said in a statement.
Crown in August posted a 7.5% fall in annual revenue to $3.34 billion, dragged down by fewer VIP players making big bets at Australian casinos.
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"If these allegations are true then Crown would be facilitating money laundering for any number of nefarious reasons like tax fraud, drug running, and even terrorism", Wilkie said.
The MP refused to reveal the former employees' level of seniority in the company, but said they were very nervous "men". When asked about the allegations earlier today, Shorten said they needed to be investigated by the Victorian regulator.
"There are gambling regulators in Victoria, and no doubt they need to investigate", he told reporters in Canberra.
Crown shares tumbled about 6.5 per cent following the airing of the allegations, falling below $11 to their lowest point in a year.
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Crown said in a statement that it rejected the allegations and that Wilkie should "immediately provide to the relevant authorities all information relating to the matters alleged".