Opener Aaron Finch will sit out the match as he continues to manage a calf injury, while allrounder Hilton Cartwright has been included despite suffering from an illness in recent days.
Australia will now face India in the first of the five ODIs at the MA Chidambaram Stadium in Chennai on Sunday.
Marcus Stoinis top-scored for Australia with 76 runs
The right-handed opener had incurred an injury in his calf muscle during the County Championships, nearly a month ago while playing for his team Surrey.
Goswami (43 in 54 balls) and Mayank Agarwal (42 in 47) had a good stand of 79 runs for the second wicket to keep the Indian side in the hunt, but the middle-order faltered against Agar's spin, and the scoreboard read 156 for 8 when Washington fell to Kane Richardson to become the paceman's second scalp.
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Marcus Stoinis, who hasn't played worldwide cricket since the series against New Zealand in February in which he hit an unbeaten 146, albeit in a losing cause, smashed five maximums as he and Matthew Wade powered Australia to 347/7. Karnewar, who plays for Vidarbha, is an ambidextrous spinner and is known to outfox batsmen in domestic circuit with his variations.
Temperatures in the low 30s and humidity of up to 80 per cent are set to test the stamina of the Australian players for the day game, which will get underway at 10am local time (2.30pm AEST). He missed the training session on Monday and final call on his selection will be taken on Tuesday morning.
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"It's tough conditions here".
Even though both the batsmen fell on reaching their respective half-century, the innings was carry forwarded by Travis Head (65) and Marcus Stoinis (76) as Australia crossed the 200-run mark.
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Australia T20 squad: Steve Smith (c), David Warner, Jason Behrendorff, Dan Christian, Nathan Coulter-Nile, Patrick Cummins, Aaron Finch, Travis Head, Moises Henriques, Glenn Maxwell, Tim Paine, Kane Richardson, Adam Zampa. However, Finch will be back for the first ODI game against Virat Kohli's men at the same venue.